Methods of producing and characterizing virus vaccine and virus vaccine composition
AssigneesGuangzhou Realbenefitspot Pharmaceutical Co., Ltd., Guangzhou Yinheyangguang Biologics Co., Ltd.
InventorsDianlian Liu, Wen Ai, Mingfeng Shen
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Abstract
This application pertains to methods of isolating virus particles and producing virus vaccine composition comprising subject a biological sample to an anion exchange chromatography and a hydroxyapatite chromatography. The application also pertains to rabies virus vaccine compositions and methods of assessing suitability of a virus vaccine composition or releasing a commercial batch of virus vaccine composition for clinical use.
Claims31
A virus composition comprising inactivated rabies virus particles, wherein at least about 90% of the total proteins in the composition are viral proteins,
- wherein the viral proteins comprise:
- at least about 35-48% protein G.
A virus composition comprising inactivated rabies virus particles comprising viral proteins, wherein the virus composition has at least about 80% purity of the rabies virus, wherein the purity is calculated by totaling proportions of all viral proteins based upon silver staining of SDS-PAGE analysis of the virus composition, wherein the viral proteins comprise:
- at least about 35-48% protein G.
A virus composition comprising inactivated rabies virus particles comprising viral proteins,
- wherein the total viral proteins comprise 35-48% protein G and 13-16% protein M.
A virus composition produced by combining a virus stock solution with a stabilizer, wherein the stock solution comprises rabies virus particles,
- wherein at least about 90% of the total proteins in the stock solution are viral proteins,
- wherein the viral proteins comprise:
- at least about 35-48% protein G.
Dependencies are inferred from the claim text.