Nanobarcode for controlling cell adhesion and differentiation of stem cells, preparation method thereof, and method of controlling adhesion and differentiation of stem cells by using the same
AssigneeKorea University Research and Business Foundation
InventorsHeemin Kang, Young Keun Kim, Sunhong Min, Yoo Sang Jeon, Hyojun Choi
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Abstract
Disclosed are a nanobarcode for controlling adhesion and differentiation of stem cells and a method of controlling adhesion and differentiation of stem cells by using nanobarcodes. The method of controlling adhesion and differentiation of stem cells of the present invention may efficiently control adhesion and differentiation of stem cells in vivo or in vitro by tuning periodicity and sequences of a ligand peptide (RGD) of a nanobarcode.
Claims8
A method of preparing a nanobarcode for controlling adhesion and differentiation of stem cells, the method comprising:
- preparing a nanobarcode in which a first segment including iron (Fe) and a second segment including gold (Au) are repeatedly formed;
- substituting a carboxylate substituent on the first segment by mixing the nanobarcode and a first suspension;
- and
- mixing the nanobarcode and a second suspension including an integrin ligand peptide (RGD), wherein the peptide binds to the second segment of the nanobarcode,
- wherein the nanobarcode satisfies Equation 1 below,
- [L(M1M2)q] [Equation 1]
- wherein, M1 is the first segment, M2 is the second segment, q is the number of times of repetition of the first and second segments, q is an integer between 2 and 10, and L is lengths of the first and second segments.
A method of controlling adhesion and differentiation of stem cells, the method comprising:
- manufacturing a nanobarcode-presenting substrate by putting a substrate of which a surface is activated in a solution containing a nanobarcode for controlling adhesion and differentiation of stem cells;
- and
- treating the stem cells with the nanobarcode-presenting substrate,
- wherein the treating of the stem cells controls adhesion and differentiation of the stem cells;
- and
- wherein the nanobarcode for controlling adhesion and differentiation of stem cells comprises:
- a nanobarcode in which a first segment including iron (Fe) and a second segment including gold (Au) are repeatedly formed;
- and
- an integrin ligand peptide bound to the second segment of the nanobarcode.
Dependencies are inferred from the claim text.