Mammalian cell population and medicaments for cell therapies in canines and improved cell cultivation methods
AssigneeScarcell Technologies
InventorsAntoine Lafont, Mathieu Castela
Life of this patent
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Abstract
Compositions of matter, including cell populations and medicaments, are provided that are derived from canine gingival fibroblasts and have cell phenotypes that occur in proportions not found in natural gum tissue, but rather, are preferentially are selected to express proteins favoring angiogenesis and anti-inflammatory effects, while reducing cell populations that promote tumorigenicity and/or formation of metalloproteinases that inhibit tissue regeneration. Methods of generating such compositions are provided that increase proliferation many-fold compared to previously known methods, and methods of using such compositions in a wide range of canine cell therapies are provided.
Claims16
A pharmaceutical composition made by a process comprising the steps of:
- obtaining canine gingival fibroblast cells representative of a naturally occurring population of canine gingival fibroblast cells in canine gum tissue;
- culturing the canine gingival fibroblast cells through four passages in a culture medium comprising 20% Fetal Bovine Serum (FBS) and Basic Fibroblast Growth Factor (bFGF);
- during a fifth passage of the process, omitting the 20% FBS from the culture medium and adding exogeneous Interleukin 1 Beta (IL-1β) in a concentration from 0.1 ng/ml to 10 ng/ml, wherein by completion of the fifth passage the canine gingival fibroblast cells correspond to cell phenotypes cultivated so that at least about 90% of the cells of the cultivated population express CD90 and CD63 and about 20% or less of the cells of the cultivated population express CD146, and
- collecting the culture medium having a protein expression produced by the fifth passage for use as the pharmaceutical composition.
A pharmaceutical composition made by a process comprising the steps of:
- obtaining canine gingival fibroblast cells representative of a naturally occurring population of canine gingival fibroblast cells in canine gum tissue;
- culturing the canine gingival fibroblast cells through four passages in a culture medium comprising 20% Fetal Bovine Serum (FBS) and Basic Fibroblast Growth Factor (bFGF);
- during a fifth passage of the process, omitting the 20% FBS and adding exogenous Interleukin 1 Beta (IL-13) in a concentration from 0.5 ng/ml to 2 ng/ml to the culture medium, wherein by completion of the fifth passage the canine gingival fibroblast cells correspond to cell phenotypes cultivated so that at least about 50% of the cells of the cultivated population express CD90 and CD63 and about 10% or less of the cells of the cultivated population express CD146;
- and
- collecting the culture medium having a protein expression produced by the fifth passage for use as the pharmaceutical composition.
A pharmaceutical composition made by a process comprising the steps of:
- obtaining canine gingival fibroblast cells representative of a naturally occurring population of canine gingival fibroblast cells in canine gum tissue;
- culturing the canine gingival fibroblast cells through four passages in a culture medium comprising 20% Fetal Bovine Serum (FBS) and Basic Fibroblast growth factor (bFGF);
- during a fifth passage of the process, omitting the 20% FBS from the culture medium and adding exogeneous Interleukin 1 Beta (IL-1B) in a concentration from 0.1 ng/ml to 10 ng/ml, wherein by completion of the fifth passage the canine gingival fibroblast cells correspond to cell phenotypes cultivated so that at least about 90% of the cells of the cultivated population express CD90 and CD63 and about 20% or less of the cells of the cultivated population express CD146, and
- collecting the culture medium having a protein expression produced by the fifth passage and adding at least one pharmaceutically acceptable carrier or excipient.
Dependencies are inferred from the claim text.