Compositions and methods for driving T1 event diversity

At least one expression cassette for producing at least 10 unique edits in a plant's T1 seed, the expression cassette comprising:

• a nucleic acid that encodes a DNA modification enzyme,
• a nucleic acid that encodes at least one guide RNA (gRNA), and
• a floral mosaic (FMOS) promoter,
• wherein the FMOS promoter
• (i) mediates expression of the DNA modification enzyme in at least one of a floral primordia cell and a floral reproductive organ, and
• (ii) mediates a plurality of edits in the at least one of the floral primordia and the floral reproductive organ, and
• (iii) mediates at least one of at least 2×, at least 3×, at least 4×, at least 5×, at least 6×, at least 7×, at least 8×, at least 9×, at least 10×, at least 11×, at least 12×, at least 13×, at least 14×, at least 15×, at least 16×, at least 17×, at least 18×, at least 19×, at least 20×, at least 21×, at least 22×, at least 23×, at least 24×, at least 25×, at least 26×, at least 27×, at least 28× at at least 27×, at least 28×, at least 29×, at least 30×, at least 31×, at least 32×, at least 33×, at least 34×, at least 35×, at least 36×, at least 37×, at least 38×, at least 39×, at least 40×, at least 41×, at least 42×, at least 43×, at least 44×, at least 45×, at least 46×, at least 47×, at least 48×, at least 49×, at least 50×, at least Six, at least 52×, at least 53×, at least 54×, at least 55×, at least 56×, at least 57×, at least 58×, at least 59×, at least 60×, at least 61×, at least 62×, at least 63×, at least 64×, at least 65×, at least 66×, at least 67×, at least 68×, at least 69×, at least 70×, at least 71×, at least 72×, at least 73×, at least 74×, at least 75×, at least 76×, at least 77×, at least 78×, at least 79×, at least 80×, at least 81×, at least 82×, at least 83×, at least 84×, at least 85×, at least 86×, at least 87×, at least 88×, at least 89×, at least 90×, at least 91×, at least 92×, at least 93×, at least 94×, at least 95×, at least 96×, at least 97×, at least 98×, at least 99× and at least 100× more expression of the DNA modification enzyme in the at least one of the floral primordia and the floral reproductive organ than in a vegetative tissue,
• and at least one of at least 2×, at least 3×, at least 4×, at least 5×, at least 6×, at least 7×, at least 8×, at least 9×, at least 10×, at least lix, at least 12×, at least 13×, at least 14×, at least 15×, at least 16×, at least 17×, at least 18×, at least 19×, at least 20×, at least 21×, at least 22×, at least 23×, at least 24×, at least 25×, at least 26×, at least 27×, at least 28× at least 27×, at least 28×, at least 29×, at least 30×, at least 31×, at least 32×, at least 33×, at least 34×, at least 35×, at least 36×, at least 37×, at least 38×, at least 39×, at least 40×, at least 41×, at least 42×, at least 43×, at least 44×, at least 45×, at least 46×, at least 47×, at least 48×, at least 49×, at least 50×, at least S1×, at least 52×, at least 53×, at least 54×, at least 55×, at least 56×, at least 57×, at least 58×, at least 59×, at least 60×, at least 61×, at least 62×, at least 63×, at least 64×, at least 65×, at least 66×, at least 67×, at least 68×, at least 69×, at least 70×, at least 71×, at least 72×, at least 73×, at least 74×, at least 75×, at least 76×, at least 77×, at least 78×, at least 79×, at least 80×, at least 81×, at least 82×, at least 83×, at least 84×, at least 85×, at least 86×, at least 87×, at least 88×, at least 89×, at least 90×, at least 91×, at least 92×, at least 93×, at least 94×, at least 95×, at least 96×, at least 97×, at least 98×, at least 99× and at least 100× more expression of the DNA modification enzyme in the at least one of the floral primordia and the floral reproductive organ than in a seed;
• and
• wherein the FMOS promoter is selected from the group consisting of SEQ ID NO:
• 2, SEQ ID NO:
• 20, SEQ ID NO:
• 26, SEQ ID NO:
• 29, SEQ ID NO:
• 35, SEQ ID NO:
• 47, SEQ ID NO:
• 50, SEQ ID NO:
• 53, SEQ ID NO:
• 56, SEQ ID NO:
• 62, SEQ ID NO:
• 65, SEQ ID NO:
• 71, SEQ ID NO:
• 74, SEQ ID NO:
• 77, SEQ ID NO:
• 80, SEQ ID NO:
• 83, SEQ ID NO:
• 86, SEQ ID NO:
• 514, SEQ ID NO:
• 518, SEQ ID NO:
• 614, SEQ ID NO:
• 615, SEQ ID NO:
• 616, SEQ ID NO:
• 617, SEQ ID NO:
• 618, SEQ ID NO:
• 619, SEQ ID NO:
• 620, SEQ ID NO:
• 621, SEQ ID NO:
• 622, SEQ ID NO:
• 698, SEQ ID NO:
• 700, SEQ ID NO:
• 702, SEQ ID NO:
• 704, SEQ ID NO:
• 706, SEQ ID NO:
• 708,SEQ ID NO:
• 710, SEQ ID NO:
• 717, SEQ ID NO:
• 719, SEQ ID NO:
• 721, and SEQ ID NO:
• 723 or a sequence with at least 95% sequence identity thereto.