Cell culture bioreactor
AssigneeABEC, Inc.
InventorsShane Alexander Jaques Kilpatrick, Scott Raymond Pundsack, Moin Ahmed
Life of this patent
Estimating the term…
Abstract
A cell culture bioreactor has perfusion membranes and gas transfer membranes or a gas phase in an extra-membrane space in contact with a film on the perfusion membranes. Gas transfer membranes may travel through the perfusion membranes or through the extra-membrane space. Examples with hollow fiber and flat sheet membranes are shown. One or more of the membranes optionally has a responsive surface, for example a thermo-responsive surface. In some examples, membranes are located in X-Y planes while the length of the reactor extends in a Z-direction.
Claims18
A cell culture bioreactor comprising,
- a plenum to contain a growth media and growing cells;
- a plurality of perfusion membranes within the plenum;
- a plurality of gas transfer membranes within the plenum;
- a plurality of potting blocks having a plurality of surfaces;
- a plurality of braces;
- and,
- a plurality of headers and/or manifolds,
- wherein the potting blocks include perfusion membrane potting blocks and gas transfer potting blocks,
- wherein the perfusion membranes are sealed in the perfusion membrane potting blocks with open ends of the perfusion membranes exposed and the gas transfer membranes are sealed in the gas transfer potting blocks with open ends of the gas transfer membranes exposed,
- wherein the membranes are sealed the plurality of potting blocks with open ends of the membranes exposed,
- wherein the braces define portions of the surfaces of the potting blocks,
- wherein the braces are stacked in a direction perpendicular to the membranes,
- wherein the membranes are arranged in layers of the perfusion membranes and layers of the gas transfer membranes,
- and wherein the headers and/or manifolds are attached to sides of the bioreactor and each header and/or manifold communicates with the open ends of the membranes of either a plurality of the layers of the perfusion membranes or a plurality of the layers of the gas transfer membranes.
Dependencies are inferred from the claim text.