Condensed polycyclic conjugated polymers and their use for biological detection

A conjugated polymer of Formula 1 comprising a conjugated segment composed of x distinct comonomer units (Mr-1 to Mr-x) that are randomly distributed along the conjugated polymer backbone:

• wherein:
• Mr-1 is of formula:
• wherein:
• the Mr-1 comonomer units are connected to adjacent comonomers of the conjugated polymer backbone through any two positions of C2, C3, C6 and C7 of the Mr-1 comonomer;
• each R is independently selected from hydrogen, alkyl, substituted alkyl, a water solubilizing group (WSG), a linker, a linked WSG (L-WSG), a linked functional group (L-FG) and a linked biological substrate (L-BS);
• Mr-2 to Mr-x are distinct comonomer units each independently selected from ethenylene, acetylene, an aryl, a heteroaryl, and a fluorescent dye (FD);
• m1 is an integer greater than or equal to 5;
• and
• m2 to mx are each independently an integer from 0 to 200;
• wherein:
• (1) the sum of m1 through mx is ≥10;
• and
• (2) at least one comonomer of Mr-1 comprises a water soluble group (WSG);
• and
• (3) at least one comonomer of Mr-2 is represented by the following structure:
• wherein:
• X is FG or L-BS;
• and
• m2 is an integer from 1 to 200.