Methods for increasing the capacity of purification processes
AssigneeEMD Millipore Corporation
InventorsVenkatesh Natarajan, Herbert Lutz, Bala Raghunath
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Abstract
In various embodiments, the present invention provides a process for separating target proteins from non-target proteins in a sample comprising increasing the concentration of the target proteins and non-target proteins in the sample and subsequently delivering the concentrated sample to a chromatography device. In other embodiments, the invention relates to a process for increasing the capacity of a chromatography device for a target protein by delivering a concentrated sample comprising the target protein to a chromatography device.
Claims7
A process for purifying target proteins from non-target proteins in a liquid sample, comprising, performing in the order recited:
- a) increasing a concentration of target proteins and non-target proteins in the liquid sample by a single-pass tangential flow filtration (SPTFF) process to produce a concentrated feed having a protein concentration of 5 g/L or greater;
- b) delivering the concentrated feed to a protein A chromatography device, wherein the SPTFF process provides direct flow-through concentration of the liquid sample to the protein A chromatography device;
- c) separating the target proteins from the non-target proteins in the protein A chromatography device;
- d) recovering the target proteins from the protein A chromatography device in a recovered sample;
- and,
- e) determining, that the capacity of the protein A chromatography device is increased to about 10 kg to 15 kg per liter of chromatography media.
Dependencies are inferred from the claim text.