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US 12,296,285 B2Granted
Methods for purifying antibodies
AssigneeUcb Biopharma Srl
InventorsNeil Alan Watson, Curtis William Phippen
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Abstract
The invention relates to the field of manufacturing recombinant antibody molecules. In particular, methods of purifying such recombinant antibody molecules are provided wherein imidazole or an imidazole-analogue is added during the elution of the recombinant antibody molecule from an affinity chromatography resin, such as a protein-A-based resin.
Claims7
A method for purifying an antibody comprising:
- a) loading a mixture comprising the antibody to be purified onto an affinity chromatography resin,
- wherein the affinity chromatography resin is selected from:
- a protein A chromatography resin, a protein G chromatography resin or a protein L chromatography resin;
- b) washing the chromatographic resin with a wash buffer;
- and
- c) eluting the antibody with an elution buffer that comprises 0.01M to 1.0M imidazole or an imidazole-analogue and a pH of 3 to 5, wherein the imidazole analogue is selected from 4 (5)-methylimidazole, pyrazole, 1,5-dimethyl-1H-pyrazole, 1,3-dimethyl-1H-pyrazole or 1H-Imidazole-1-ylmethanol.
3 more elements
Dependencies are inferred from the claim text.