Fusion proteins for hydroxylating amino acids and products
AssigneeHtl Biotechnology Innovation, Inc.
InventorsLixin Dai, Poonam Srivastava
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Abstract
The disclosure herein provides a fusion protein comprising prolyl 4-hydroxylase alpha subunit (P4HA) and a soluble protein partner. A fusion protein comprising prolyl 4-hydroxylase alpha subunit (P4HA1) and prolyl 4-hydroxylase beta subunit (P4HB) is provided. A microorganism including a fusion protein comprising prolyl 4-hydroxylase alpha subunit-1 (P4HA1) and prolyl 4-hydroxylase beta subunit (P4HB) is provided. The disclosure provides a microorganism including a fusion protein comprising prolyl 4-hydroxylase alpha subunit-1 (P4HA1) and prolyl 4-hydroxylase beta subunit (P4HB); and another protein to be hydroxylated. A method for providing skincare benefits including applying the fusion protein of the present disclosure on skin is also taught.
Claims3
A fusion protein comprising:
- a prolyl 4-hydroxylase alpha subunit;
- and
- a prolyl 4-hydroxylase beta subunit,
- wherein the prolyl 4-hydroxylase alpha subunit is at the N-terminus of the fusion protein and the prolyl 4-hydroxylase beta subunit is at the C-terminus of the fusion protein, wherein the prolyl 4-hydroxylase alpha subunit is encoded by a polynucleotide comprising SEQ ID NO:
- 1.
A fusion protein comprising:
- a prolyl 4-hydroxylase alpha subunit;
- and
- a prolyl 4-hydroxylase beta subunit,
- wherein the prolyl 4-hydroxylase alpha subunit is at the N-terminus of the fusion protein and the prolyl 4-hydroxylase beta subunit is at the C-terminus of the fusion protein, wherein the prolyl 4-hydroxylase beta subunit is encoded by a polynucleotide comprising SEQ ID NO:
- 2.
A fusion protein comprising:
- a prolyl 4-hydroxylase alpha subunit;
- and
- a prolyl 4-hydroxylase beta subunit,
- wherein the prolyl 4-hydroxylase alpha subunit is at the N-terminus of the fusion protein and the prolyl 4-hydroxylase beta subunit is at the C-terminus of the fusion protein, wherein the prolyl 4-hydroxylase alpha subunit is encoded by a polynucleotide comprising SEQ ID NO:
- 1 and the prolyl 4-hydroxylase beta subunit is encoded by a polynucleotide comprising SEQ ID NO:
- 2.
Dependencies are inferred from the claim text.