Methods and compositions related to modified green fluorescent protein (GFP) with an embedded foreign peptide
AssigneeUniversity of South Florida
InventorsVladimir N. Uversky, Bogdan S. Melnik
Life of this patent
Estimating the term…
Abstract
Disclosed herein are methods and compositions comprising chimeric green fluorescent protein (GFP) molecules embedded with foreign molecules. Also disclosed are methods of treating a subject with chimeric GFP molecules embedded with foreign molecules. Further disclosed are methods of delivering a desired molecule to a cell, wherein said desired molecule is embedded within a chimeric GFP molecule.
Claims4
A composition comprising a modified green fluorescent protein (GFP) molecule and a bactenecin, wherein the bactenecin consists of SEQ ID NO:
- 1 and replaces at least a part of a central α-helix region of a GFP molecule comprising SEQ ID NO:
- 2, and wherein the modified GFP molecule comprises the amino acid substitutions F8D, I167D, and Q177E, which confer stability to the composition.
A nucleic acid encoding a modified GFP molecule and a bactenecin, wherein the bactenecin consists of SEQ ID NO:
- 1 and replaces at least a part of a central α-helical moiety of a GFP molecule comprising SEQ ID NO:
- 2, and wherein the modified GFP molecule comprises the amino acid substitutions G8D, I167D, and Q177E, which confer stability to the composition.
Dependencies are inferred from the claim text.