Method for identification of characteristic components in Kai-Xin-San by thin-layer chromatography (TLC)

A method for identification of characteristic components in a Kai-Xin-San by thin-layer chromatography (TLC), comprising the following steps:

• collecting a first test solution of Kai-Xin-San, a first negative test solution of Kai-Xin-San, a first reference medicinal material solution, and a first standard solution, then spotting on a first silica gel G thin-layer plate to determine characteristic components in ginseng radix et rhizoma and polygalae radix, respectively;
• collecting a second test solution of Kai-Xin-San, a second negative test solution of Kai-Xin-San, a second reference medicinal material solution, and a second standard solution, then spotting on a second silica gel G thin-layer plate to determine characteristic components in Poria and Acori tatarinowii Rhizoma, respectively;
• developing the first silica gel G thin-layer plate with a developing solvent of dichloromethane (DCM)-n-butanol (n-BuOH)-water, developing the second silica gel G thin-layer plate with a developing solvent of DCM-ethyl acetate-formic acid, then taking out the first silica gel G thin-layer plate from the developing solvent and the second silica gel G thin-layer plate from the developing solvent to allow air-drying after the developing is completed;
• wherein DCM, n-BuOH, and water in the developing solvent of DCM-n-BuOH-water are at a volume ratio of 7-8:4:1, and DCM, ethyl acetate, and formic acid in the developing solvent of DCM-ethyl acetate-formic acid are at a volume ratio of 12:3:0.25-0.5;
• and
• spraying the first air-dried silica gel G thin-layer plate with a sulfuric acid-ethanol solution for color development inspection, and spraying the second air-dried silica gel G thin-layer plate with a vanillin sulfuric acid-ethanol mixed solution for color development inspection.