Fluorescence observation apparatus, fluorescence observation system, and fluorescence observation method

A fluorescence observation system, comprising:

• a fluorescence observation apparatus that comprises:
• an irradiation unit configured to apply a plurality of kinds of excitation light of mutually different wavelengths to a plurality of spatially or temporally different positions in a biological sample that is labeled with a composite phosphor, wherein the composite phosphor includes two or more kinds of fluorescent molecules at a specific composition ratio;
• and
• a detection unit configured to detect fluorescence generated at each of the plurality of spatially or temporally different positions, wherein the fluorescence is generated based on the application of the plurality of kinds of excitation light by the irradiation unit;
• and
• a processing unit configured to process a detection result of the detection unit, wherein
• the processing unit is executed by the fluorescence observation apparatus, and
• the processing unit is configured to determine a distribution of pieces of the composite phosphor based on a fluorescence signal of each piece of the detected fluorescence, wherein
• the fluorescence signal is obtained based on the detection result of the detection unit, and
• the fluorescence signal shows a fluorescence intensity corresponding to a position of the plurality of spatially or temporally different positions in the biological sample.

A fluorescence observation method, comprising:

• applying a plurality of kinds of excitation light of mutually different wavelengths to a plurality of spatially or temporally different positions in a biological sample that is labeled with a composite phosphor, wherein the composite phosphor includes or more kinds of fluorescent molecules at a predetermined specific composition ratio;
• detecting fluorescence generated at each of the plurality of spatially or temporally different positions, wherein the fluorescence is generated based on the application of the plurality of kinds of excitation light;
• and
• determining a distribution of pieces of the composite phosphor based on a fluorescence signal of each piece of the detected fluorescence, wherein
• the fluorescence signal is obtained based on a result of the detecting, and
• the fluorescence signal shows a fluorescence intensity corresponding to a position of the plurality of spatially or temporally different positions in the biological sample.