Modified yeast and method for increasing lysine content in fermentation co-products
AssigneeDanisco US Inc.
InventorsJaclyn Diana Demartini, Celia Emily Gaby Payen
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Abstract
Described are strains and methods relating to genetically-engineered yeast cells that overproduce lysine in a tunable manner by altering feedback inhibition of the lysine synthetic pathway by way of the LYS20 and LYS21 homocitrate synthase polypeptides. The yeast can be used in a conventional bioethanol production facility to produce alcohol along with increased amounts of lysine, resulting in increased quality and commercial value of fermentation products and co-products, such as animal feed ingredients.
Claims9
A non-naturally-occurring variant homocitrate synthase polypeptide having at least 80% amino acid sequence identity to SEQ ID NO:
- 2 and comprising one or more mutations selected from the group consisting of F36Y, N38K, N289D, R349K, Q352E, V375D, R376T and I380M, with respect to SEQ ID NO:
- 2, wherein the variant homocitrate synthase polypeptide demonstrates reduced lysine inhibition compared to an otherwise identical homocitrate synthase polypeptide lacking the one or more mutations.
Dependencies are inferred from the claim text.