Cell culture container having minute volume

A method for manufacturing a cell culture container which comprises a step of bringing a coating agent into contact with a surface of a cell culture container having at least one opened minute space which has a volume of 20 μL or less, to form a hydrophilic coating film having a difference between the maximum film thickness and the minimum film thickness of 1,000 Å or less onto the surface portion of the container constituting the minute space,

• wherein the coating agent contains a copolymer having the recurring units represented by formulae (a1) and (b1):
• wherein Ta, Tb, Ua1, Ua2, Ub1, Ub2 and Ub3 each independently represent a hydrogen atom or a linear or branched alkyl group having 1 to 5 carbon atoms, Qa and Qb represent an ester bond, Ra and Rb each independently represent a linear or branched alkylene group having 1 to 18 carbon atoms which may be substituted by halogen atom(s), An− represents an anion selected from the group consisting of a halide ion, an inorganic acid ion, a hydroxide ion and an isothiocyanate ion, and m represents an integer of 0 to 6,
• wherein the coating agent contains (i) the copolymer with a solid content of 0.01 to 4% by mass and (ii) water as a solvent, and
• wherein the hydrophilic coating film has no bubble therein and has a surface with a water contact angle in air of 0° to 120° and a bubble contact angle in water of 80° to 180°.