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US 12,291,562 B2Granted
Reagents and assays using modified integrin domains
AssigneesRush University Medical Center, 149 Bio, LLC
InventorsVineet Gupta, Antonio J. Barbosa
Life of this patent
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Abstract
Modified integrin polypeptides are provided. Methods of identifying binding agents that bind to a modified integrin polypeptide are also provided.
Claims9
A modified integrin polypeptide comprising at least one amino acid substitution in a metal ion binding site, and at least one conformation specific amino acid substitution selected from the group consisting of F156A, F275S, F275R, F302L and F302W of a wild type integrin polypeptide;
- wherein the wild type integrin polypeptide comprises SEQ ID NO:
- 1;
- and
- wherein the metal ion binding site is selected from the group consisting of metal ion dependent adhesion site (MIDAS), adjacent to MIDAS (ADMIDAS) and ligand-associated metal binding site (LIMBS)/synergistic metal binding site (SyMBS).
1 more element
Dependencies are inferred from the claim text.