Modified activin A
AssigneesMitsubishi Chemical Corporation, UniBio Corporation
InventorsHidehito Takayama, Risa Watanabe, Yutaka Takemoto, Hiroyuki Tanaka, Hiroshi Kawabata, Kyoji Yoshinaka
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Abstract
The purpose of the present invention is to provide a modified activin A. The present invention provides activin A comprising a modified proregion.
Claims12
A recombinant activin A, comprising a modified proregion, wherein the modified proregion comprises an amino acid sequence selected from the group consisting of (a), (b) and (d):
- (a) an amino acid sequence in which an amino acid sequence from position 182 to 199 of the amino acid sequence of SEQ ID NOs:
- 4, 5 or 6 is deleted;
- (b) an amino acid sequence in which an amino acid sequence from position 182 to 199 of the amino acid sequence of SEQ ID NOs:
- 4, 5 or 6 is substituted with a spacer sequence consisting of 1 to 10 amino acids;
- and
- (d) an amino acid sequence in which a least one of amino acids from position 180 to 201 of the amino acid sequence of SEQ ID NOs:
- 4, 5, or 6 has at least one amino acid inserted therein.
A recombinant activin A, comprising a modified proregion, wherein the modified proregion comprises an amino acid sequences selected from the group consisting of (e) to (g):
- (e) an amino acid sequence selected from the group consisting of the amino acid sequence of SEQ ID NOs:
- 9 to 20;
- (f) an amino acid sequence in which at least one amino acid is deleted, substituted or added in an amino acid sequence selected from the group consisting of the amino acid sequence of SEQ ID NOs:
- 9 to 20, wherein the recombinant activin A whose proregion is modified so that it has resistance to degradation by a protease other than Furin;
- and
- (g) an amino acid sequence having at least 80% homology with an amino acid sequence selected from the group consisting of the amino acid sequence of SEQ ID NOs:
- 9 to 20, wherein the recombinant activin A comprising the amino acid sequence has resistance to degradation by a protease other than Furin.
A mixture comprising:
- (i) a recombinant activin A comprising a modified proregion and
- (ii) a recombinant mature activin A,
- wherein the modified proregion comprises an amino acid sequence selected from the group consisting of (a), (b), and (d):
- (a) an amino acid sequence in which an amino acid sequence from position 182 to 199 of the amino acid sequence of SEQ ID NOs:
- 4, 5, or 6 is deleted;
- (b) an amino acid sequence in which an amino acid sequence from position 182 to 199 of the amino acid sequence of SEQ ID NOs:
- 4, 5, or 6 is substituted with a spacer sequence consisting of 1 to 10 amino acids;
- and
- (d) an amino acid sequence in which an amino acid sequence from position 180 to 201 of the amino acid sequence of SEQ ID NOs:
- 4, 5, or 6 has at least one amino acid inserted therein.
Dependencies are inferred from the claim text.