Hydrolyzed compound protein for larval largemouth bass (Micropterus salmoides) and use thereof

A compound protein for larval largemouth bass (Micropterus salmoides), comprising the following components:

• 72-74 parts by weight of a hydrolyzed protein pre-mixture, 3-5 parts by weight of mixed fish soluble meal, 4-6 parts by weight of gluten flour, 2-4 parts by weight of sodium caseinate, and 0.4-0.6 parts by weight of a yeast hydrolysate, wherein the compound protein for larval largemouth bass is obtained by fully pulverizing the components, passing the components through a 100-mesh sieve, and then fully mixing in proportion the components, and wherein the hydrolyzed protein pre-mixture comprises white fish meal, fermented soybean meal, shrimp meal and blood meal, and the weight ratio is 58-62:5-7:4-6:2-3, respectively;
• wherein the hydrolyzed protein pre-mixture is prepared by a method comprising the following steps:
• a) mixing the white fish meal, the fermented soybean meal, the shrimp meal, and the blood meal to obtain a premix, and then mixing the premix with sterile distilled water in a solid-liquid ratio of 1:2;
• b) adding in 2 wt. % of a neutral protease and 1 wt. % of a keratinase into the premix, mixing evenly, and then stirring to form an enzymatic premixture;
• c) heating the enzymatic premixture in b) into a 50° C. water bath for 30 min, and stirring the enzymatic premixture once every 10 min;
• d) putting an enzymatic hydrolysate into the enzymatic premixture of c) and then air drying at 105° C. for sterilization and enzyme inactivation for 30 min to obtain a resulting product;
• and
• e) then drying the resulting product after the sterilization and the enzyme inactivation in d) at 80° C. for 12 h, and then obtaining the hydrolyzed protein pre-mixture.