Methods for making high intensity sweeteners
AssigneeFirmenich SA
InventorsAndrew P. Patron, Chris Edano Noriega, Rama Rao Manam, Justin Colquitt, Nathan Faber, Helge Zieler, Justin Stege, Daniel K. Olson
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Abstract
Provided herein include methods of making mogroside compounds, e.g., Compound 1, compositions (for example, host cells) for making mogroside compounds, and the mogroside compounds made by the methods and compositions disclosed herein, compositions made by the methods (for example, cell lysates), and recombinant cells comprising the mogroside compounds (e.g., Compound 1).
Claims20
A method of producing Compound 1 having the structure of:
- the method comprising:
- contacting mogroside IIIE with a recombinant host cell that comprises a first enzyme capable of catalyzing a production of Compound 1 from mogroside IIIE, wherein the recombinant host cell comprises:
- (1) a cytochrome P450 that comprises an amino acid sequence having at least 90% sequence identity to SEQ ID NO:
- 1025;
- and/or
- (2) a cytochrome P450 that is encoded by a nucleic acid sequence having at least 90% sequence identity to SEQ ID NO:
- 1024;
- and/or
- (3) a glycosylating enzyme that comprises an amino acid sequence having at least 90% sequence identity to SEQ ID NO:
- 1051;
- and/or
- (4) a glycosylating enzyme that is encoded a nucleic acid sequence having at least 90% sequence identity to SEQ ID NO:
- 1050;
- and/or
- (5) an epoxide hydrolase that comprises an amino acid sequence having at least 90% sequence identity to the sequence of SEQ ID NO:
- 1073;
- and/or
- (6) an epoxide hydrolase that is encoded by a nucleic acid sequence having at least 90% sequence identify to the sequence of SEQ ID NO:
- 1072.
A recombinant cell comprising:
- Compound 1 having the structure:
- a gene encoding an enzyme capable of catalyzing production of Compound 1 from mogroside IIIE, and one or more of
- (1) a cytochrome P450 that comprises an amino acid sequence having at least 90% sequence identity to SEQ ID NO:
- 1025;
- and/or
- (2) a cytochrome P450 that is encoded by a nucleic acid sequence having at least 90% sequence identity to SEQ ID NO:
- 1024;
- and/or
- (3) a glycosylating enzyme that comprises an amino acid sequence having at least 90% sequence identity to SEQ ID NO:
- 1051;
- and/or
- (4) a glycosylating enzyme that is encoded a nucleic acid sequence having at least 90% sequence identity to SEQ ID NO:
- 1050;
- and/or
- (5) an epoxide hydrolase that comprises an amino acid sequence having at least 90% sequence identity to the sequence of SEQ ID NO:
- 1073;
- and/or
- (6) an epoxide hydrolase that is encoded by a nucleic acid sequence having at least 90% sequence identify to the sequence of SEQ ID NO:
- 1072.
A recombinant cell comprising a first gene encoding a first enzyme capable of catalyzing production of Compound 1 having the structure:
- from mogroside IIIE, and one or more of
- (1) a cytochrome P450 that comprises an amino acid sequence having at least 90% sequence identity to SEQ ID NO:
- 1025;
- and/or
- (2) a cytochrome P450 that is encoded by a nucleic acid sequence having at least 90% sequence identity to SEQ ID NO:
- 1024;
- and/or
- (3) a glycosylating enzyme that comprises an amino acid sequence having at least 90% sequence identity to SEQ ID NO:
- 1051;
- and/or
- (4) a glycosylating enzyme that is encoded a nucleic acid sequence having at least 90% sequence identity to SEQ ID NO:
- 1050;
- and/or
- (5) an epoxide hydrolase that comprises an amino acid sequence having at least 90% sequence identity to the sequence of SEQ ID NO:
- 1073;
- and/or
- (6) an epoxide hydrolase that is encoded by a nucleic acid sequence having at least 90% sequence identify to the sequence of SEQ ID NO:
- 1072.
Dependencies are inferred from the claim text.