Microbial fermentation for the production of terpenes
AssigneeLanzaTech, Inc.
InventorMichael Koepke
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Abstract
The invention provides a method for producing a terpene or a precursor thereof by microbial fermentation. Typically, the method involves culturing a recombinant bacterium in the presence of a gaseous substrate whereby the bacterium produces a terpene or a precursor thereof, such as mevalonic acid, isopentenyl pyrophosphate, dimethylallyl pyrophosphate, isoprene, geranyl pyrophosphate, farnesyl pyrophosphate, and/or farnesene. The bacterium may comprise one or more exogenous enzymes, such as enzymes in mevalonate, DXS, or terpene biosynthesis pathways.
Claims2
A method for producing isoprene by providing at least one C1 compound selected from the group consisting of carbon monoxide and carbon dioxide into contact with a recombinant C1-fixing microorganism comprising a nucleic acid encoding a group of exogenous enzymes comprising thiolase, 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) synthase, and HMG-CoA reductase, mevalonate kinase, phosphomevalonate kinase, mevalonate diphosphate decarboxylase, isopentenyl-diphosphate delta-isomerase, and isoprene synthase, wherein the microorganism is Clostridium or Moorella, to allow the microorganism to produce isoprene from the C1 compound, and converting the isoprene into synthetic rubber tires.
Dependencies are inferred from the claim text.