Methods of altering seed weight and seed oil content by manipulating alpha-carboxyl-transferase (a-CT) activity via carboxyl-transferase interaction (CTI) protein expression
AssigneeThe Curators of the University of Missouri
InventorsJay Thelen, Yajin Ye
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Abstract
A method of altering fatty acid and/or triacylglycerol production in plants and/or algae is provided. The method includes altering activity levels of alpha-carboxyltransferase (α-CT), a catalytic subunit of acetyl-CoA carboxylase (ACCase) by modulating an expression of at least one carboxyltransferase interaction (CTI) gene encoding at least one CTI protein.
Claims16
A method of increasing fatty acid and/or triacylglycerol production in plants and/or algae, comprising genetically modifying the plants and/or algae to increase activity levels of alpha-carboxyltransferase (α-CT) comprising the step of decreasing intracellular concentrations of one or more carboxyl transferase interactor (CTI) proteins with a polypeptide sequence having at least 95% sequence identity to a polypeptide sequence selected from the group consisting of SEQ ID NOs:
- 2, 4, and 6, and wherein the one or more CTI proteins inhibit activity levels of α-CT, wherein α-CT comprises a catalytic subunit of acetyl-CoA carboxylase (ACCase).
A genetically modified plant having an increased activity level of alpha-carboxyltransferase (α-CT) and a decreased expression of one or more CTI genes in comparison to a wild-type plant of the same species grown under the same conditions, wherein α-CT comprises a catalytic subunit of acetyl-CoA carboxylase (ACCase), wherein the one or more CTI genes comprise a sequence having at least 95% sequence identity to a nucleotide sequence selected from the group consisting of SEQ ID NOs:
- 1, 3, 5, or a complement thereof, or wherein the one or more CTI genes encode a CTI protein with a polypeptide sequence having at least 95% sequence identity to a polypeptide sequence selected from the group consisting of SEQ ID NOs:
- 2, 4, and 6.
Dependencies are inferred from the claim text.