Back to results
US 12,286,643 B2Granted
Methods for generating hematopoietic stem cells
AssigneeThe Brigham and Women's Hospital, Inc.
InventorDhvanit I. Shah
Life of this patent
Estimating the term…
Abstract
In some aspects and embodiments, the invention provides methods for making hematopoietic stem cells, including for HSCT. The method comprises providing a cell population comprising hemogenic endothelial (HE) or endothelial cells, and increasing activity or expression of DNA (cytosine-5-)-methyltransferase 3 beta (Dnmt3b) and/or GTPase IMAP Family Member 6 (Gimap6) in the HE and/or endothelial cells under conditions sufficient for stimulating formation of HSCs.
Claims20
A method of preparing a population of hematopoietic stem cells (HSCs), the method comprising:
- providing a population comprising hemogenic endothelial (HE) cells, the population provided by isolating CD34+cells from embryoid bodies, the embryoid bodies being differentiated from human induced pluripotent stem cells (iPSCs);
- and
- contacting the population comprising HE cells with an effective amount of Yoda 1 in the absence of a transgene and under conditions sufficient for stimulating formation of HSCs.
Dependencies are inferred from the claim text.