Lactic acid bacteria
AssigneeInternational N&H Denmark Aps
InventorsArmelle Cochu-Blachére, Christophe Fremaux, Thomas Desfougéres, Anaïs Jedrzejowski
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Abstract
The invention relates to a lactose-positive, galactose-negative, Streptococcus thermophilus strain carrying a mutation in 2 or 3 genes selected from the group consisting of 1) a gene encoding a protein of the mannose-glucose-specific PTS and a glcK gene, 2) a gene encoding a protein of the mannose-glucose-specific PTS and a ccpA gene, and 3) a gene encoding a protein of the mannose-glucose-specific PTS, a glcK gene and a ccpA gene, wherein said strain, when used to ferment milk, provides a low lactose fermented milk and/or a fermented milk not undergoing acidification when stored at fermentation temperature.
Claims15
A lactose-positive, galactose-negative, Streptococcus thermophilus strain comprising:
- at least one mutated gene encoding a protein of the mannose-glucose-specific phosphotransferase system (mannose-glucose-specific PTS), the glucose import activity of which is decreased or abolished, and
- at least one mutated gene selected from the group consisting of:
- a) a mutation in a glucokinase (glcK) gene such that the glucokinase activity of said strain is reduced but not null,
- wherein the protein sequence of said glucokinase is a glcK variant sequence having at least 90% identity with SEQ ID NO:25 or SEQ ID NO:46,
- wherein the amino acid of the glucokinase corresponding to position 275 of SEQ ID NO:
- 25 is not a glutamic acid, and/or the amino acid of the glucokinase corresponding to position 144 of SEQ ID NO:46 is not a glycine;
- b) a mutation in a catabolite control protein A (ccpA) gene, wherein the sequence of said mutated ccpA gene is selected from the group consisting of:
- i) a sequence comprising SEQ ID NO:71, and
- ii) a ccpA variant sequence having at least 90% identity with SEQ ID NO:71;
- and
- c) a combination thereof.
Dependencies are inferred from the claim text.