Scaffold proteins
AssigneeAvacta Life Sciences Limited
InventorsGeoffrey William Platt, Graham Robert Pye-Smith Spence
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Abstract
The invention relates to a polypeptide, such as an Affimer polypeptide, comprising an amino acid sequence having at least 80% identity to amino acid residues 1 to 11, 13 to 15, 17 to 19, 21 to 25, 27 to 28, 35 to 37, 39, 41, 43 to 44, 46 to 47, 49 to 50, 52 to 53, 55 to 58, 63 to 64, 66, 68 to 82, 84 to 85, and 87 to 98 of SEQ ID NO: 1; characterized in that said polypeptide comprises one or mutations relative to SEQ ID NO: 1. The invention also relates to various methods and nucleic acids.
Claims19
A nucleic acid comprising a nucleotide sequence encoding a polypeptide, wherein the polypeptide comprises a scaffold protein sequence, wherein the scaffold protein sequence (a) comprises a N32G mutation relative to the amino acid sequence of SEQ ID NO:
- 1 and (b) has at least 90% identity to the amino acid sequence of SEQ ID NO:
- 1, and
- the polypeptide further comprises a first heterologous peptide and a second heterologous peptide inserted in the scaffold protein sequence, wherein the first heterologous peptide and second heterologous peptides are excluded from the at least 90% identity calculation.
A nucleic acid comprising a nucleotide sequence encoding a polypeptide, wherein the polypeptide comprises a scaffold protein sequence, wherein the scaffold protein sequence (a) comprises N32G, Y35W, Q42E, V48D, T51L, and M65I mutations relative to the amino acid sequence of SEQ ID NO:
- 1 and (b) has at least 80% identity to the amino acid sequence of SEQ ID NO:
- 1, and the polypeptide further comprises a first heterologous peptide and a second heterologous peptide inserted in the scaffold protein sequence, wherein the first heterologous peptide and second heterologous peptides are excluded from the at least 80% identity calculation.
Dependencies are inferred from the claim text.