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US 12,286,467 B2Granted
Cell culture media containing combinations of proteins
AssigneeInvitria, Inc.
InventorsSteven Clyde Pettit, Mary Ann Michelle Fernandez Santos, Ning Huang
Life of this patent
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Abstract
The present invention relates to methods of enhancing transfection in cell culture media supplemented with a plant-produced recombinant mammalian transferrin supplement, as well as kits, and methods of using the supplemented cell culture media to improve growth characteristics of cultured cells for transfection.
Claims13
A method of enhancing transfection efficiency of a cell population during transfection, said method comprising:
- a) transfecting a cell population with a nucleic acid molecule in a cell culture media supplemented with a plant-produced recombinant mammalian transferrin related protein, wherein said nucleic acid molecule comprises an expression vector comprising a nucleotide sequence encoding a protein of interest,
- wherein said transfection efficiency of said cell population is enhanced relative to a control cell population transfected in cell culture media without said plant-produced recombinant mammalian transferrin related protein supplement.
Dependencies are inferred from the claim text.