Steviol glycoside transport
AssigneeDsm IP Assets B.V.
InventorsViktor Marius Boer, Priscilla Zwartjens, Eric Van Den Berg
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Abstract
A recombinant host capable of producing a steviol glycoside which overexpresses a polypeptide which mediates steviol glycoside transport and which polypeptide comprises the amino acid sequence set forth in SEQ ID NO: 29 or an amino acid sequence having at least about 50% sequence identity thereto. A recombinant host capable of producing a steviol glycoside which has been modified, preferably in its genome, to result in a deficiency in the production of a polypeptide which mediates steviol glycoside transport and which polypeptide comprises the amino acid sequence set forth in SEQ ID NO: 29 or an amino acid sequence having at least about 50% sequence identity thereto.
Claims13
A recombinant host capable of producing a steviol glycoside which overexpresses a polypeptide which mediates steviol glycoside transport and which polypeptide comprises the amino acid sequence set forth in SEQ ID NO:29 or an amino acid sequence having at least about 95% sequence identity thereto, wherein the recombinant host comprises one or more recombinant nucleic acid sequence encoding:
- (i) a polypeptide having UDP-glycosyltransferase 74G1 (UGT74G1) activity;
- (ii) a polypeptide having UDP-glycosyltransferase 2 (UGT2) activity;
- (iii) a polypeptide having UDP-glycosyltransferase 85C2 (UGT85C2) activity;
- and
- (iv) a polypeptide having UDP-glycosyltransferase 76G1 (UGT76G1) activity.
A recombinant host capable of producing a steviol glycoside which overexpresses a polypeptide which mediates steviol glycoside transport and which polypeptide comprises the amino acid sequence set forth in SEQ ID NO:29 or an amino acid sequence having at least about 95% sequence identity thereto, wherein the recombinant host comprises one or more recombinant nucleic acid sequence encoding:
- (i) a polypeptide having UDP-glycosyltransferase 74G1 (UGT74G1) activity;
- (ii) a polypeptide having UDP-glycosyltransferase 2 (UGT2) activity;
- (iii) a polypeptide having UDP-glycosyltransferase 85C2 (UGT85C2) activity;
- (iv) a polypeptide having UDP-glycosyltransferase 76G1 (UGT76G1) activity;
- (v) a polypeptide having ent-copalyl pyrophosphate synthase activity;
- (vi) a polypeptide having ent-Kaurene synthase activity;
- (vii) a polypeptide having ent-Kaurene oxidase activity;
- (viii) a polypeptide having kaurenoic acid 13-hydroxylase activity.
Dependencies are inferred from the claim text.