Methods and compositions related to improved nitrogen utilization efficiency in tobacco
AssigneeAltria Client Services LLC
InventorsJesse Frederick, Chengalrayan Kudithipudi, Dongmei Xu
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Abstract
The present disclosure provides metabolic signatures and genetic markers for tracking enhanced nitrogen utilization efficiency phenotypes in tobacco plants and for introgressing enhanced nitrogen utilization efficiency phenotypes into tobacco plants. The disclosure also provides tobacco plants comprising enhanced nitrogen utilization efficiency and methods to the creation of tobacco plants comprising enhanced nitrogen utilization efficiency. The disclosure also provides recombinant polynucleotides and polypeptides for enhancing nitrogen utilization efficiency in modified tobacco plants and tobacco plants comprising the provided recombinant polynucleotides and polypeptides.
Claims10
A method of producing a Nicotiana tabacum plant comprising an enhanced nitrogen utilization efficiency (NUE) trait, said method comprising:
- a) selecting a Nicotiana tabacum plant comprising at least one favorable NUE allele comprising a T nucleotide at a position corresponding to position 36 of SEQ ID NO:
- 61 or a T nucleotide at a position corresponding to position 36 of SEQ ID NO:
- 64;
- b) crossing said Nicotiana tabacum plant selected in step a) with a second Nicotiana tabacum plant;
- and
- c) obtaining progeny seed from a cross of step b) wherein a plant grown from said progeny seed comprises said enhanced NUE trait and said at least one favorable NUE allele.
A method of introgressing an enhanced nitrogen utilization efficiency (NUE) trait into a Nicotiana tabacum variety, said method comprising the steps of:
- a) crossing a tobacco plant from a first Nicotiana tabacum variety comprising an enhanced NUE trait with a tobacco plant from a second Nicotiana tabacum variety lacking said enhanced NUE trait;
- b) obtaining progeny seed from the cross of step a);
- and
- c) selecting at least one progeny seed comprising at least one favorable NUE allele comprising a T nucleotide at a position corresponding to position 36 of SEQ ID NO:
- 61 or a T nucleotide at a position corresponding to position 36 of SEQ ID NO:
- 64 and wherein a plant grown from said at least one progeny seed further comprises said enhanced NUE trait.
Dependencies are inferred from the claim text.