Method of producing N-acyl-amino group-containing compound
AssigneeAjinomoto Co., Inc.
InventorsHidemi Nagata, Keiko Danjou, Jun Takakura, Hiroyuki Nozaki
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Abstract
A method efficiently produces a compound containing an N-acyl-amino group by an enzymatic method. Specifically, a method of producing a compound containing an N-acyl-amino group includes producing the compound containing an N-acyl-amino group by reacting a compound containing an amino group with a compound containing a carboxyl group in the presence of an enzyme having an ability to bond a carboxyl group and an amino group in an ATP dependent manner to form an amide bond.
Claims14
A method of producing a compound containing an N-acyl-amino group, the method comprising:
- reacting a compound containing an amino group with a fatty acid in a culture medium comprising a microorganism, wherein the microorganism produces an enzyme, and said reacting results in production of the compound containing an N-acyl-amino group, wherein the fatty acid is a saturated fatty acid having 6 to 18 carbon atoms, and
- isolating the compound containing an N-acyl-amino group from the culture medium,
- wherein the enzyme has an ability to bond a carboxyl group and an amino group in an ATP dependent manner to form an amide bond, wherein the enzyme is selected from the group consisting of:
- (A) a protein comprising an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 1 to 9,
- (B) a protein comprising an amino acid sequence containing one to twenty amino acid substitutions, deletions, insertions, or additions in the amino acid sequence selected from the group consisting of SEQ ID NOs:
- 1 to 9, and said protein having an N-acylase activity, and
- (C) a protein comprising an amino acid sequence having 95% or more identity to the amino acid sequence selected from the group consisting of SEQ ID NOs:
- 1 to 9, and said protein having an N-acylase activity.
A method of producing a compound containing an N-acyl-amino group, the method comprising:
- reacting a compound containing an amino group with a fatty acid in a culture medium comprising a microorganism, wherein the microorganism produces an enzyme, and said reacting results in production of the compound containing an N-acyl-amino group, and
- isolating the compound containing an N-acyl-amino group from the culture medium,
- wherein the enzyme has an ability to bond a carboxyl group and an amino group in an ATP dependent manner to form an amide bond,
- wherein the enzyme is selected from the group consisting of:
- (A) a protein comprising an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 1 to 9,
- (B) a protein comprising an amino acid sequence containing one to twenty amino acid substitutions, deletions, insertions, or additions in the amino acid sequence selected from the group consisting of SEQ ID NOs:
- 1 to 9, and said protein having an N-acylase activity, and
- (C) a protein comprising an amino acid sequence having 95% or more identity to the amino acid sequence selected from the group consisting of SEQ ID NOs:
- 1 to 9, and said protein having an N-acylase activity;
- wherein the fatty acid is selected from the group consisting of caproic acid (C6), enanthic acid (C7), caprylic acid (C8), pelargonic acid (C9), capric acid (C10), undecylic acid (C11), lauric acid (C12), tridecylic acid (C13), myristic acid (C14), pentadecylic acid (C15), palmitic acid, palmitoleic acid, sapienic acid (C16), margaric acid (C17), stearic acid, α-linolenic acid, γ-linolenic acid, linoleic acid, vaccenic acid, oleic acid (C18), coconut oil fatty acid, palm fatty acid, hardened beef tallow fatty acid, and combinations thereof.
Dependencies are inferred from the claim text.