Back to results
US 12,276,671 B2Granted
Phospho-TAU antibodies and methods of use
AssigneeAlzpath, Inc.
InventorsAndreas Jeromin, Krish Venkat
Life of this patent
Estimating the term…
Abstract
Provided herein are compositions and methods relating to improved assays for establishing Alzheimer's disease. Further provided herein are compositions and methods comprising improved antibodies for assays including immunoassays.
Claims48
A method for detecting phosphorylated tau in a sample from an individual comprising:
- performing an immunoassay on the sample using an antibody or antibody fragment comprising a variable domain, heavy chain region (VH) and a variable domain, light chain region (VL), wherein:
- (a) the VH comprises SEQ ID NO:
- 31 and the VL comprises SEQ ID NO:
- 36;
- (b) the VH comprises SEQ ID NO:
- 33 and the VL comprises SEQ ID NO:
- 39;
- (c) the VH domain comprises SEQ ID NO:
- 34 and the VL comprises SEQ ID NO:
- 40;
- (d) the VH domain comprises SEQ ID NO:
- 30 and the VL comprises SEQ ID NO:
- 35;
- (e) the VH domain comprises SEQ ID NO:
- 31 and the VL comprises SEQ ID NO:
- 37;
- or
- (f) the VH domain comprises SEQ ID NO:
- 32 and the VL comprises SEQ ID NO:
- 38.
17 more elements
A method for detecting phosphorylated tau in a sample from an individual comprising:
- performing an immunoassay on the sample using an antibody or antibody fragment comprising a variable domain, heavy chain region (VH) and a variable domain, light chain region (VL),
- wherein the VH comprises an amino acid sequence at least about 90% identical to a sequence as set forth in any one of SEQ ID NOs:
- 30-34, and wherein the VL comprises an amino acid sequence at least about 90% identical to a sequence as set forth in any one of SEQ ID NOS:
- 35-40;
- and
- wherein the VH comprises a HCDR1 sequence, a HCDR2 sequence, and a HCDR3 sequence, and the VL comprises a LCDR1 sequence, a LCDR2 sequence, and a LCDR3 sequence, wherein:
- (a) the HCDR1 sequence comprises SEQ ID NO:
- 2, the HCDR2 sequence comprises SEQ ID NO:
- 7, the HCDR3 sequence comprises SEQ ID NO:
- 11, the LCDR1 sequence comprises SEQ ID NO:
- 15, the LCDR2 sequence comprises SEQ ID NO:
- 21, and the LCDR3 sequence comprises SEQ ID NO:
- 25;
- (b) the HCDR1 sequence comprises SEQ ID NO:
- 4, the HCDR2 sequence comprises SEQ ID NO:
- 7, the HCDR3 sequence comprises SEQ ID NO:
- 12, the LCDR1 sequence comprises SEQ ID NO:
- 18, the LCDR2 sequence comprises SEQ ID NO:
- 23, and the LCDR3 sequence comprises SEQ ID NO:
- 28;
- (c) the HCDR1 sequence comprises SEQ ID NO:
- 5, the HCDR2 sequence comprises SEQ ID NO:
- 9, the HCDR3 sequence comprises SEQ ID NO:
- 13, the LCDR1 sequence comprises SEQ ID NO:
- 19, the LCDR2 sequence comprises SEQ ID NO:
- 21, and the LCDR3 sequence comprises SEQ ID NO:
- 29;
- (d) the HCDR1 sequence comprises SEQ ID NO:
- 1, the HCDR2 sequence comprises SEQ ID NO:
- 6, the HCDR3 sequence comprises SEQ ID NO:
- 10, the LCDR1 sequence comprises SEQ ID NO:
- 14, the LCDR2 sequence comprises SEQ ID NO:
- 20, and the LCDR3 sequence comprises SEQ ID NO:
- 24;
- (e) the HCDR1 sequence comprises SEQ ID NO:
- 2, the HCDR2 sequence comprises SEQ ID NO:
- 7, the HCDR3 sequence comprises SEQ ID NO:
- 11, the LCDR1 sequence comprises SEQ ID NO:
- 16, the LCDR2 sequence comprises SEQ ID NO:
- 22, and the LCDR3 sequence comprises SEQ ID NO:
- 26;
- or
- (f) wherein the HCDR1 sequence comprises SEQ ID NO:
- 3, the HCDR2 sequence comprises SEQ ID NO:
- 8, the HCDR3 sequence comprises SEQ ID NO:
- 10, the LCDR1 sequence comprises SEQ ID NO:
- 17, the LCDR2 sequence comprises SEQ ID NO:
- 20, and the LCDR3 sequence comprises SEQ ID NO:
- 27.
46 more elements
A method for detecting phosphorylated tau in a sample from an individual comprising:
- performing an immunoassay on the sample using an antibody comprising a heavy chain and a light chain, wherein:
- (a) the heavy chain comprises SEQ ID NO:
- 43 and the light chain comprises SEQ ID NO:
- 44;
- (b) the heavy chain comprises SEQ ID NO:
- 48 and the light chain comprises SEQ ID NO:
- 49;
- (c) the heavy chain comprises SEQ ID NO:
- 50 and the light chain comprises SEQ ID NO:
- 51;
- (d) the heavy chain comprises SEQ ID NO:
- 41 and the light chain comprises SEQ ID NO:
- 42;
- (e) the heavy chain comprises SEQ ID NO:
- 43 and the light chain comprises SEQ ID NO:
- 45;
- or
- (f) the heavy chain comprises SEQ ID NO:
- 46 and the light chain comprises SEQ ID NO:
- 47.
17 more elements
Dependencies are inferred from the claim text.