Time-resolved fluorescence immunochromatography kit for simultaneous detection of mixed pollutant of aflatoxin and carbaryl, preparation method therefor, and application thereof

An immunochromatographic time-resolved fluorescence immunochromatography kit for simultaneously detecting mixed pollution of aflatoxin and carbaryl, comprising:

• an immunochromatographic time-resolved fluorescent test strip, and a sample reaction vial containing a europium-labeled anti-aflatoxin monoclonal antibody freeze-dried product and a europium-labeled anti-carbaryl monoclonal antibody freeze-dried product, wherein the immunochromatographic time-resolved fluorescent test strip includes a paperboard, an absorbent pad (1), a test pad (2) and a sample pad (3) successively stacked onto one side of the paperboard from one edge to another edge of the paperboard, wherein adjacent pads are overlapped and connected at junctions, the test pad (2) uses a nitrocellulose membrane as a base pad, a quality control line (4) and test lines (5, 6) are transversely arranged on the nitrocellulose membrane from the one edge to the another edge of the paperboard, the quality control line (4) is coated with a rabbit anti-mouse polyclonal antibody, the test lines are located below the quality control line, the number of the test lines is 2, and the test lines (5, 6) are respectively coated with a carbaryl-ovalbumin conjugate and an aflatoxin-bovine serum albumin conjugate;
• and wherein the anti-carbaryl monoclonal antibody is secreted by a hybridoma cell strain Jnw1D2 that is deposited under the terms of the Budapest Treaty in CCTCC with an accession number of CCTCC NO. C201654;
• and wherein the anti-aflatoxin monoclonal antibody is secreted from a hybridoma cell strain 1C11 that is deposited under the terms of the Budapest Treaty in CCTCC with an accession number of CCTCC NO. C201013.