Compositions and methods for passive optical barcoding for multiplexed assays
AssigneeSlingshot Biosciences, Inc.
InventorsJeffrey Kim, Anh Tuan Nguyen, Brandon Miller
Life of this patent
Estimating the term…
Abstract
Compositions comprising multiple hydrogel particles having substantially the same diameter, but with each subgrouping of particles from the multiple hydrogel particles having different associated values for one or more passive optical properties that can be deconvoluted using cytometric instrumentation. Each hydrogel particle from the multiple hydrogel particles can be functionalized with a different biochemical or chemical target from a set of targets. A method of preparing hydrogel particles includes forming droplets and polymerizing the droplets, with optional functionalization.
Claims15
A composition for demultiplexing one or more targets based on an encoded optical property, said composition comprising:
- a plurality of populations of hydrogel particles, each population of hydrogel particles having substantially the same average particle diameter,
- wherein each population of hydrogel particles comprises a different forward and/or side scatter optical property, so as to be optically distinguishable from other populations of hydrogel particles within the composition;
- wherein each population of hydrogel particles comprises a different functionalized ligand configured to form a particle target combination with a target selected from the group consisting of a chemical, cellular, or biochemical target;
- wherein each population of the plurality of hydrogel particles is configured to allow for simultaneous demultiplexing of the one or more of the target.
Dependencies are inferred from the claim text.