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US 12,275,975 B2Granted
Conversion of lignin into bioplastics and lipid fuels
AssigneeThe Texas A&MUniversity System
InventorsShuhua Yuan, Shangxian Xie, Xin Wang
Life of this patent
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Abstract
The present invention provides methods and compositions for increasing lignin degradation to produce a biological product. Also provided are methods for increasing expression of laccase in a bacterial species to produce increased lignin degradation. Also provided are bacterial cells and commodities or commodity produces produced from such methods.
Claims9
A method of lignin depolymerization and optionally production of polyhydroxyalkanoate (PHA), said method comprising
- expressing in a Rhodococcus or Pseudomonas bacterium a first expression cassette comprising a lignin depolymerization module and a second expression cassette comprising a PHA production module, wherein said lignin depolymerization module comprises a polynucleotide encoding a lignin degradation enzyme, a promoter driving expression of the lignin degradation enzyme, and a polynucleotide encoding a secretion signal peptide for the lignin degradation enzyme selected from the group consisting of OprI, OprF, Pbp and PelB, and said PHA production module comprises a PHA synthesis gene and a promoter driving expression of the PHA synthesis gene, and
- contacting the bacterium with lignin,
- wherein expression of said first and second expression cassettes results in lignin depolymerization and optionally production of PHA.
Dependencies are inferred from the claim text.