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US 12,275,974 B2Granted
Synthetic enzyme complexes for in vitro rubber production
AssigneeThe United States of America as represented by the Secretary of Agriculture
InventorsGrisel P. Ponciano, Colleen M. McMahan, Paul Hoeprich, Patrick Scannon
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Abstract
Disclosed herein are recombinant enzymes derived from various rubber-producing plants and the use of those enzymes in the production of natural rubber outside of plant tissues. Systems and methods for the utilization of these enzymes in the production of natural rubber are also provided.
Claims25
A composition for producing rubber polymer in vitro, comprising:
- a recombinant cis-prenyltransferase protein with a sequence identity at least 80% identical to SEQ ID NO:
- 2 obtained from Parthenium argentatum or with a sequence identity at least 80% identical to SEQ ID NO:
- 6 obtained from Hevea brasiliensis,
- a recombinant cis-prenyltransferase binding protein with a sequence identity at least 80% identical to SEQ ID NO:
- 4 obtained from Parthenium argentatum or a sequence identity at least 80% identical to SEQ ID NO:
- 8 obtained from Hevea brasiliensis,
- wherein both said cis-prenyltransferase and cis-prenyltransferase binding proteins are incorporated into a membrane composed of lipid wherein the lipid and proteins form a synthetic molecular complex;
- an allylic pyrophosphate reaction initiator;
- a monomer-isopentenyl pyrophosphate substrate convertible into a polymer by said cis-prenyltransferase,
- and an aqueous medium.
7 more elements
A composition for producing rubber polymer in vitro, comprising:
- a recombinant cis-prenyltransferase protein with a sequence at least 95% identical to a SEQ ID NO:
- 2 or SEQ ID NO:
- 6 incorporated into a membrane composed of lipid;
- a recombinant cis-prenyltransferase binding protein with a sequence at least 95% identical to SEQ ID NO:
- 4 or SEQ ID NO:
- 8 incorporated into a membrane composed of lipid;
- a reaction allylic pyrophosphate initiator;
- a substrate convertible into a polymer by said cis-prenyltransferase;
- and an aqueous medium.
6 more elements
Dependencies are inferred from the claim text.