US 12,275,954 B2Granted

Method of identifying l/m-opsin and CRX positive cone photoreceptor cells based on specific biomarkers

AssigneeUCL Business PLC

InventorsJane Sowden, Jorn Lakowski, Robin Ali, Emily Welby, Anai Gonzalez Cordero

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Abstract

The present invention relates to the identification of photoreceptors or cone photoreceptors in populations of cells. In particular the present invention relates to methods of identification of photoreceptors or cone photoreceptors and methods of isolating photoreceptors or cone photoreceptors. Photoreceptors or cone photoreceptors isolated by the methods of the present invention are useful for transplantation and the treatment of retinal dystrophies. Also claimed are human cell populations enriched for photoreceptors or cone photoreceptors and kits for identifying photoreceptors or cone photoreceptors.

Claims7

Dependent claims

A method of identifying L/M-Opsin and CRX positive cone photoreceptor cells in a population of cells, comprising:

a) detecting and determining whether or not cells in the population express CD29 or CD15-SSEA1 on cell surface;
b) detecting and determining whether or not cells in the population express at least two of CD26, CD133 and CD 147 on the cell surface;
and c) identifying cells as L/M-Opsin and CRX positive cone photoreceptor cells based on presence of L/M-Opsin and CRX, absence of CD29 or CD15-SSEA1, and presence of at least two of CD26, CD133 and CD147, and wherein the cells are L/M-Opsin and CRX positive, CD29 or CD15-SSEA1 negative and positive for at least two of CD26, CD133 and CD147.

Dependencies are inferred from the claim text.

Facts

Application: 16/488,380Filed 21 Feb 2018
Priority: GB 170305824 Feb 2017
Published: US 2020/0063095 A127 Feb 2020
Granted: 15 Apr 2025
Term ends: Not estimatedThe source records don’t support an estimate.

Classification12 codes

C12N 5/062
Cells of the nervous system: sensory transducers, e.g. photoreceptors; Sensory neurons, e.g. for hearing, taste, smell, pH, touch, temperature, pain
A61K 9/0048
Galenical forms characterised by the site of application: eye, e.g. artificial tears
A61K 35/30
Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically…:…
G01N 33/56966
For microorganisms, e.g. protozoa, bacteria, viruses: animal cells