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US 12,275,708 B2Granted
Hydrophilic high quantum yield acridinium esters with improved stability and fast light emission
AssigneeSiemens Healthcare Diagnostics Inc.
InventorsAnand Natrajan, David Sharpe, Qingping Jiang, David Wen
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Abstract
Hydrophilic, high quantum yield, chemiluminescent acridinium compounds with increased light output, improved stability, fast light emission and decreased non specific binding are disclosed. The chemiluminescent acridinium esters possess hydrophilic, branched, electron-donating functional groups at the C2 and/or C7 positions of the acridinium nucleus.
Claims9
An acridinium compound having the structure:
- wherein,
- R1 is a methyl or sulfopropyl group;
- R2a is alkylene or arylene,
- R3a is alkyl or aryl,
- G is, at both occurrences, a branched group selected from:
- and
- R12 is selected from the group consisting of
- (1) —OH;
- (2) —O—N-succinimidyl;
- (3) —NH—(CH2)5—C(O)—O—N-succinimidyl;
- (4) —NH—(CH2)5—COOH;
- (5) —NH—(C2H4O)p—C2H4NH—C(O)—(CH2)3—C(O)—O—N—R″ wherein p=1 to 5;
- and
- where R″ is hydrogen or —N-succinimidyl;
- and
- (6) —NH—(C2H4O)q—C2H4NH2, wherein q=1 to 5.
13 more elements
A method for the detection or quantification of an analyte in a sample suspected of containing the analyte comprising the steps of:
- (a) providing a conjugate comprising (i) an antibody specific for the analyte;
- and (ii) an acridinium compound having the structure:
- wherein,
- R1 is a methyl or sulfopropyl group;
- R2a is alkylene or arylene,
- R3a is alkyl or aryl,
- G is, at both occurrences, a branched group selected from:
- and
- R12 is conjugated to the antibody specific for the analyte;
- (b) providing a solid support having immobilized thereon a second antibody specific for the analyte;
- (c) mixing the conjugate, the solid phase and the sample suspected of containing the analyte to form a binding complex;
- (d) separating the binding complex captured on the solid support;
- (e) triggering chemiluminescence of the binding complex from step (d) by adding chemiluminescence triggering reagents;
- (f) detecting the presence or calculating the concentration of the analyte by comparing the amount of light emitted from the reaction mixture with a standard dose response curve which relates the amount of light emitted to a known concentration of the analyte.
11 more elements
Dependencies are inferred from the claim text.