US 12,274,279 B2 · Methods for isolating compounds

Abstract

The present invention in its broadest aspect relates to a method for reducing glycoalkaloid content and turbidity of an aqueous phase comprising compounds selected from two or more of PA, PI, PPO, LipO, pectin, lipid, glycoalkaloid and phenolic compounds of which at least one compound is selected from PA, PT, LipO and PPO; a) providing an aqueous phase comprising compounds selected from two or more of PA, PI, PPO, LipO, pectin, lipid, glycoalkaloid and phenolic compounds of which at least one compound is selected from PA, PT, LipO and PPO; and b) performing one or more steps to reduce the concentration of solanine in the dry matter of the aqueous phase with at least 15 percent, such as at least 20%, such as at least 25% and to achieve an optical density at 620 nm of the remaining aqueous phase of less than 0.7; such as less than 0.5; such as less than 0.3; such as less than 0.2; such as less than 0.1; and thereby obtaining an aqueous phase having reduced glycoalkaloid content and turbidity compared to an untreated aqueous phase.

Claims20

Dependent claims

A method for isolating a first group of compounds selected from one or more of patatin protein (PA), protease inhibitor protein (PI), lipoxygenase (LipO) and polyphenol oxidase (PPO) from a second group of compounds selected from one or more of PA, PI, LipO, PPO, glycoalkaloid and phenolic compounds, said method comprising:

a) providing an aqueous phase comprising compounds selected from two or more of PA, PI, PPO, LipO, glycoalkaloid and phenolic compounds of which at least one compound is selected from one or more of PA, PI, LipO and PPO;
b) contacting the aqueous phase with a mobile solubilized ligand at physico-chemical conditions allowing formation of a complex between the ligand and the compounds selected from one or more of PA, PI, LipO and PPO;
c) allowing the complex to separate from the aqueous supernatant phase, optionally by changing said physico-chemical conditions in the composition to reduce the stability of the complex;
and
d) isolating the complex separated from the aqueous supernatant phase by precipitation.

2 more elements

Dependencies are inferred from the claim text.

Methods for isolating compounds

Life of this patent

Methods for isolating compounds

Life of this patent

Abstract

Claims20