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US 12,272,048 B2Granted
Automated detection and repositioning of micro-objects in microfluidic devices
OwnerFenglei Du, Paul M. Lundquist +2inventors
InventorsFenglei Du, Paul M. Lundquist, John A. Tenney, Troy A. Lionberger
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Abstract
Methods are provided for the automated detection of micro-objects in a microfluidic device. In addition, methods are provided for repositioning micro-objects in a microfluidic device. In addition, methods are provided for separating micro-objects in a spatial region of the microfluidic device.
Claims25
A method for determining a number density of micro-objects disposed within a predetermined area of a microfluidic device, the method comprising:
- capturing a digital image of a region in the microfluidic device that contains a micro-object of interest;
- determining periodic structures in the digital image using a Fourier transform;
- generating a filtered image or a differential image by removing the periodic structures from the digital image;
- identifying a micro-object of interest based on the filtered or differential image;
- and
- calculating the number density of micro-objects in the pre-determined area of that region of the microfluidic device using the filtered or differential image.
3 more elements
Dependencies are inferred from the claim text.