Quantitation of tamoxifen and metabolites thereof by mass spectrometry
AssigneeQuest Diagnostics Investments Incorporated
InventorNigel Clarke
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Abstract
Provided are methods for determining the amount of tamoxifen and its metabolites in a sample by mass spectrometry. In some aspects, the methods provided herein determine the amount of N-Desmethyl Tamoxifen. In some aspects, the methods provided herein determine the amount of N-Desmethyl Tamoxifen and other tamoxifen metabolites. In some aspects, the methods provided herein determine the amount of tamoxifen, N-Desmethyl Tamoxifen, and other tamoxifen metabolites.
Claims9
A method for determining an amount of endoxifen and N-desmethyl-4′-hydroxy tamoxifen in a sample by a single mass spectrometry assay, the method comprising:
- purifying the sample by liquid chromatography;
- ionizing the endoxifen and N-desmethyl-4′-hydroxy tamoxifen in the sample to produce one or more ions detectable by mass spectrometry;
- and
- detecting an amount of the one or more ion(s) by mass spectrometry;
- wherein:
- the sample is a human sample;
- the amount of the one or more ion(s) detected is related to the amount of each of endoxifen and N-desmethyl-4′-hydroxy tamoxifen in the sample;
- and
- the method has a limit of quantitation of less than or equal to 0.4 ng/ml.
Dependencies are inferred from the claim text.