Fixatives and methods of use
AssigneeThe United States of America, as represented by the Secretary, Department of Health and Human Services
InventorsStephen M. Hewitt, Joon-Yong Chung, Candice D. Perry, Robert A. Star
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Abstract
Disclosed herein are compositions for fixing tissue for cytologic, histomorphologic, and/or molecular analysis (e.g., DNA, RNA, and/or protein analysis). In some embodiments, the fixatives are aldehyde-free fixatives, for example, formaldehyde- or formalin-free fixatives. Particular disclosed compositions include buffered ethanol. The buffer is a phosphate buffer or phosphate buffered saline (PBS) in some examples. In further embodiments, the fixative includes additional components, such as glycerol and/or acetic acid.
Claims19
A fixative comprising 70% ethanol, 0.5× phosphate-buffered saline, and at least one guanidinium salt.
Dependencies are inferred from the claim text.