Production method for L-cyclic amino acids
AssigneeUbe Corporation
InventorsMasaharu Mizutani, Ryoma Miyake, Hiroshi Kawabata
Life of this patent
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Abstract
An object of the present invention is to provide a method of industrially producing a high-purity L-cyclic amino acid more inexpensively and with a high efficiency, from a cyclic amino acid having a double bond at the 1-position. The present invention provides a method in which an L-cyclic amino acid is produced by allowing a cyclic amino acid having a double bond at the 1-position to react with a specific enzyme having a catalytic ability to reduce a cyclic amino acid having a double bond at the 1-position to produce an L-cyclic amino acid.
Claims7
A method of producing an L-cyclic amino acid, the method comprising bringing a cyclic amino acid having a double bond at the 1-position and having the following general formula (I):
- wherein A is an alkylene chain which has a chain length of from 1 to 4 atoms, which contains or does not contain at least one hetero atom selected from the group consisting of a sulfur atom, an oxygen atom and a nitrogen atom, in the chain or at the end of the chain, and which has or does not have a substituent,
- into contact with a polypeptide shown in (A), (B) or (C) below, a microorganism or cell having the ability to produce said polypeptide or comprising said polypeptide, a processed product of the microorganism or the cell, and/or a culture liquid obtained by culturing the microorganism or the cell and comprising said polypeptide, to produce an L-cyclic amino acid having the following general formula (II):
- wherein A is the same as defined above:
- (A) a polypeptide comprising the amino acid sequence of SEQ ID NO:
- 2, 4, 6, 8, 10 or 12;
- (B) a polypeptide which comprises the amino acid sequence of SEQ ID NO:
- 2, 4, 6, 8, 10 or 12 except that 1 to 10 amino acids are deleted, conservatively substituted and/or added, and which has the ability to catalyze the reaction of the following formula (1) to produce the L-cyclic amino acid:
- wherein A is the same as defined above;
- or
- (C) a polypeptide which comprises an amino acid sequence having a sequence identity of 95% or more to the amino acid sequence of SEQ ID NO:
- 2, 4, 6, 8, 10 or 12, and which has the ability to catalyze the reaction of the formula (1) to produce the L-cyclic amino acid.
Dependencies are inferred from the claim text.