Method for preparing an irradiated platelet lysate
AssigneeMaco Pharma
InventorsBruno Delorme, Sabrina Viau
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Abstract
The invention relates to a method for preparing an irradiated platelet lysate, comprising the steps of providing a starting platelet lysate having platelet factors including growth factors and plasma proteins including coagulation factors and proteins other than the coagulation factors. Double irradiation of the starting platelet lysate, using UVC radiation and ionizing radiation. The double irradiation with UVC radiation and ionizing radiation being arranged to retain at least 75% of the total protein concentration of the starting platelet lysate, while reducing, by at least 40%, the concentration of at least one of the coagulation factors including fibrinogen, factor II, factor VII, factor IX, factor X and factor XI of the starting platelet lysate.
Claims19
A method for preparing an irradiated platelet lysate comprising the following steps:
- providing a platelet lysate to obtain a starting platelet lysate, the starting platelet lysate comprising platelet factors including growth factors and further comprising plasma proteins including a plurality of coagulation factors and proteins other than the coagulation factors;
- subjecting the starting platelet lysate to double irradiation with UVC radiation having a wavelength between 200 and 280 nm and with ionizing radiation having a wavelength less than or equal to 100 nm to obtain a platelet lysate irradiated with UVC radiation and with ionizing radiation,
- the double irradiation with UVC radiation and with ionizing radiation being arranged to retain at least 75% of the total protein concentration of the starting platelet lysate while reducing by at least 40% the concentration of at least one of the coagulation factors selected from the group consisting of fibrinogen, factor II, factor VII, factor IX, factor X, and factor XI of the starting platelet lysate.
Dependencies are inferred from the claim text.