Soybean lines with low saturated fatty acid and high oleic acid contents
AssigneeBoard of Trustees of Southern Illinois University
InventorsKhalid Meksem, Naoufal Lakhssassi, Dounya Knizia, Zhou Zhou
Life of this patent
Estimating the term…
Abstract
The present invention is directed to a transgenic soybean plant having increased oleic acid content comprising a polynucleotide comprising a fatty acid thioesterase (FAT) related promoter that functions in the soybean plant operably linked to a polynucleotide encoding a polypeptide having FAT activity. The invention is further directed to a method of increasing oleic acid content of a soybean plant comprising transforming a soybean plant with a polynucleotide comprising a fatty acid thioesterase (FAT) related promoter that functions in the soybean plant operably linked to a polynucleotide encoding a polypeptide having FAT activity.
Claims5
A transgenic soybean plant having increased oleic acid content transformed with a polynucleotide encoding a fatty acid thioesterase (FAT) related promoter that functions in the soybean plant operably linked to a polynucleotide encoding a polypeptide having FAT activity,
- wherein the polypeptide having FAT activity comprises a sequence at least 95% identical to wild type “Forrest” FATB2A amino acid sequence (SEQ ID NO:
- 32) and further comprises one or more mutations selected from the group consisting of:
- P16L, A373T, R385Q, and G395D, and
- wherein the FAT related promoter comprises a sequence selected from the group consisting of wild type “Forrest” FATA1A (SEQ ID NO:
- 17), wild type “Forrest” FATB1A (SEQ ID NO:
- 21), wild type “Forrest” FATB1B (SEQ ID NO:
- 25), wild type “Forrest” FATB2A (SEQ ID NO:
- 29), and wild type “Forrest” FATB2B (SEQ ID NO:
- 33) nucleotide sequence.
A method of increasing oleic acid content of a soybean plant, the method comprising:
- transforming a soybean plant with a polynucleotide encoding a fatty acid thioesterase (FAT) related promoter that functions in the soybean plant operably linked to a polynucleotide encoding a polypeptide having FAT activity,
- wherein the polypeptide having FAT activity comprises a sequence at least 95% identical to wild type “Forrest” FATB2A amino acid sequence (SEQ ID NO:
- 32) and further comprises one or more mutations selected from the group consisting of:
- P16L, A373T, R385Q, and G395D, and
- wherein the FAT related promoter comprises a sequence selected from the group consisting of wild type “Forrest” FATA1A (SEQ ID NO:
- 17), wild type “Forrest” FATB1A (SEQ ID NO:
- 21), wild type “Forrest” FATB1B (SEQ ID NO:
- 25), wild type “Forrest” FATB2A (SEQ ID NO:
- 29), and wild type “Forrest” FATB2B (SEQ ID NO:
- 33) nucleotide sequence.
Dependencies are inferred from the claim text.