US 12,270,031 B2 · Polypeptide combination used for tumour immunotherapy, and preparation method therefor

Abstract

Provided is a polypeptide combination, a targeting component thereof comprising a shielding peptide, a cleavable part, an antigen-binding part and a first intein fragment, the shielding peptide and the antigen-binding part being connected by means of the cleavable part, and the antigen-binding part being directly or indirectly connected to the first intein fragment; a toxin component thereof comprises a second intein fragment and a toxin, and the second intein fragment is directly or indirectly connected to the toxin; the targeting component and the toxin component form an immunoconjugate by means of the interactive action between the first intein fragment and the second intein fragment; in the immunoconjugate, the shielding peptide and the antigen-binding part are connected by means of the cleavable part, and the antigen-binding part is connected to the toxin. Also provided are a preparation method and a pharmaceutical use for the polypeptide combination.

Claims20

Dependent claims

A polypeptide combination, which includes a targeting component and a toxin component, wherein:

said targeting component includes a shielding peptide, a cleavable part, an antigen-binding part, and a first intein fragment, said shielding peptide and said antigen-binding part are connected by said cleavable part, and said antigen-binding part is directly or indirectly connected with said first intein fragment;
said toxin component comprises a second intein fragment and a toxin, and said second intein fragment is directly or indirectly connected to said toxin;
said targeting component and said toxin component are able to form an immunoconjugate through an interaction between said first intein fragment and said second intein fragment;
said immunoconjugate comprises said shielding peptide, said antigen-binding part and said toxin, wherein said shielding peptide is connected with said antigen-binding part through said cleavable part, and said antigen-binding part is directly or indirectly connected with said toxin.

1 more element

A method for preparing an immunoconjugate, comprising the following steps:

1) Providing a targeting component, said targeting component comprises a shielding peptide, a said cleavable part, an antigen-binding part and a first intein fragment, said shielding peptide is connected with said antigen-binding part through said cleavable part, and said antigen-binding part is directly or indirectly connected with said first intein fragment;
2) Providing a toxin component comprising a second intein fragment and a toxin, and said second intein fragment is directly or indirectly connected with said toxin;
3) Contacting said targeted component and said toxin component under the condition that said first intein fragment and said second intein fragment are able to interact, wherein said targeted component and said toxin component form said immunoconjugate through an interaction between said first intein fragment and said second intein fragment.

Dependencies are inferred from the claim text.

Polypeptide combination used for tumour immunotherapy, and preparation method therefor

Life of this patent

Polypeptide combination used for tumour immunotherapy, and preparation method therefor

Life of this patent

Abstract

Claims20