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US 12,269,852 B2Granted
Processes for producing a fermentation product
AssigneeNovozymes A/S
InventorsMarc Dominique Morant, Tomoko Matsui, Kirk Matthew Schnorr, Shiro Fukuyama, Noriko Tsutsumi, Zhengfang Kang
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Abstract
The present invention relates to polypeptides having trehalase activity, particularly derived from Talaromyces. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides for the production of ethanol.
Claims12
A process of producing ethanol, comprising
- (a) liquefying a starch-containing material with an alpha-amylase to form a liquefied material;
- (b) saccharifying the liquefied material;
- (c) fermenting using a fermentation organism;
- wherein a glucoamylase and a polypeptide having trehalase activity and at least 75% sequence identity to the mature polypeptide of SEQ ID NO:
- 23 are present and/or added during saccharification step (b) or fermentation step (c) and wherein sequence identity is determined using the Needleman-Wunsch algorithm as implemented in the Needle program of the EMBOSS package, version 5.0.0, using a gap open penalty of 10, a gap extension penalty of 0.5, and EBLOSUM62 substitution matrix.
2 more elements
Dependencies are inferred from the claim text.