Method of in situ remediation of soils using a bioremediation composition including a time release material

A method of in situ remediation of contaminated media, comprising:

• forming a bioremediation composition for remediation of soil, sludge, wastewater, or groundwater containing contaminants by mixing:
• (a) a first bioremediation material comprising at least one organism capable of degrading a first contaminant;
• (b) a second bioremediation material comprising at least one organism capable of degrading a second contaminant;
• and (c) a time release substrate combined with the first and second bioremediation materials;
• forming an aqueous suspension of the bioremediation composition;
• and
• introducing into the contaminated media the aqueous suspension of the bioremediation composition,
• wherein the time release substrate includes an organic compound comprising a polysaccharide, the polysaccharide excluding cold water soluble starches and a third bioremediation material, mixed with the organic compound,
• wherein the third bioremediation material comprises at least one organism capable of degrading the organic compound for use by the organisms of at least one of the first and second bioremediation materials during the degrading of the first and second contaminants,
• wherein the degrading of the organic compound by the organisms of the third bioremediation material is performed over a time period of at least 20 days,
• wherein the first bioremediation material further comprises an adsorbent capable of adsorbing hydrocarbons and a compound that releases sulfate over a period of time, and
• wherein the first bioremediation material comprises a nutrient system for promoting growth of microorganisms capable of metabolizing the adsorbed hydrocarbons.

A method of in situ remediation of contaminated media, comprising:

• preparing a bioremediation composition by mixing:
• (a) a first bioremediation composition comprising a first blend of one or more organisms capable of degrading hydrocarbons;
• (b) a second bioremediation composition comprising a second blend of one or more organisms differing from the first blend of one or more organisms and being capable of degrading at least one halogenated compound;
• (c) an organic compound comprising a polysaccharide, the polysaccharide excluding cold water soluble starches;
• and (d) a third blend of one or more organisms capable of degrading the organic compound;
• and
• introducing the bioremediation composition into the contaminated media,
• wherein the degrading of the organic compound by the third blend of one or more organisms comprises, over a time period of at least 20 days, breaking the organic compound into a plurality of smaller molecules utilized by the second blend of one or more organisms of the second bioremediation composition during the degrading of the at least one halogenated compound,
• wherein the first bioremediation composition further comprises an adsorbent capable of adsorbing hydrocarbons and a compound that releases sulfate over a period of time,
• wherein the first blend of one or more organisms comprises a nutrient system for promoting growth of microorganisms capable of metabolizing the hydrocarbons, and
• wherein the adsorbent comprises activated carbon.