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US 12,269,036 B2Granted
Devices, systems, and methods for increasing droplet formation efficiency
Assignee10x Genomics, Inc.
InventorsIvan Akhremichev, Rajiv Bharadwaj, Lynna Chen, Mohammad Rahimi Lenji, Alireza Salmanzadeh, Martin Sauzade, Tobias Daniel Wheeler, Yiran Zhang
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Abstract
Devices, systems, and their methods of use, for generating and collecting droplets are provided. The device includes a collection region comprising a side wall canted at an angle. The invention further provides multiplex devices that increase droplet formation.
Claims20
A device for producing droplets of a first liquid in a second liquid, the device comprising:
- a) a sample inlet;
- b) first, second, and third sample channels, each of which is in fluid communication with the sample inlet;
- c) first and second reagent inlets;
- d) first, second, and third reagent channels, wherein the first and second reagent channels are in fluid communication with the first reagent inlet;
- the third reagent channel is in fluid communication with the second reagent inlet;
- the first sample channel and the first reagent channel intersect at a first intersection, the second sample channel and the second reagent channel intersect at a second intersection, and the third sample channel and the third reagent channel intersect at a third intersection;
- e) first, second, and third droplet formation regions;
- and
- f) first and second collection reservoirs,
- wherein the first droplet formation region is fluidically disposed between the first intersection and the first collection reservoir, the second droplet formation region is fluidically disposed between the second intersection and the first collection reservoir, and the third droplet formation region is fluidically disposed between the third intersection and the second collection reservoir.
7 more elements
A device for producing droplets, the device comprising:
- a) a sample inlet;
- b) first, second, third, and fourth sample channels, each of which is in fluid communication with the sample inlet;
- c) first and second reagent inlets;
- d) first, second, third, and fourth reagent channels, wherein the first and second reagent channels are in fluid communication with the first reagent inlet;
- wherein the third and fourth reagent channels are in fluid communication with the second reagent inlet;
- wherein the first sample channel and the first reagent channel intersect at a first intersection, the second sample channel and the second reagent channel intersect at a second intersection, the third sample channel and the third reagent channel intersect at a third intersection, and the fourth sample channel and the fourth reagent channel intersect at a fourth intersection;
- e) first, second, third, and fourth droplet formation regions;
- and
- f) a collection reservoir,
- wherein the first droplet formation region is fluidically disposed between the first intersection and the collection reservoir, the second droplet formation region is fluidically disposed between the second intersection and the collection reservoir, the third droplet formation region is fluidically disposed between the third intersection and the collection reservoir, and the fourth droplet formation region is fluidically disposed between the fourth intersection and the collection reservoir.
7 more elements
Dependencies are inferred from the claim text.