Colloidal particles functionalized homogeneously by biomolecules

A method for obtaining a colloid comprising functionalized liquid colloidal particles comprising the following steps:

• a) dispersing an oil in an aqueous solution comprising a fragmentation surfactant, leading to obtaining an emulsion comprising oil droplets suspended in an aqueous phase;
• b) dissolving lipids aimed at functionalizing the oil droplets in a polar aprotic solvent, leading to obtaining a functionalization solution;
• c) preparing a functionalization mixture comprising the emulsion and the functionalization solution, the volume fraction of the polar aprotic solvent in the functionalization mixture being comprised between 1 and 15%;
• d) incubating the functionalization mixture, during which at least a part of the lipids initially present in the functionalization solution are adsorbed on the surface of the oil droplets initially present in the emulsion;
• and
• e) eliminating non-adsorbed lipids during step d);
• thus allowing to obtain a colloid comprising functionalized liquid colloidal particles consisting of the oil droplets obtained after step a) on the surface of which the lipids are adsorbed during step d).