Method for producing sulfated polysaccharide and method for producing PAPS

A method for producing a sulfated polysaccharide, the method comprising the following steps (1-1) and (1-2):

• (1-1) preparing a transformant (a) of a bacterium of the genus Corynebacterium, comprising at least a gene encoding an adenosine 5′-triphosphate (ATP) sulfurylase and a gene encoding an adenosine 5′-phosphosulfate (APS) kinase which are introduced thereinto in an expressible manner and in which gene expression of an enzyme related to degradation of 3′-phosphoadenosine 5′-phosphosulfate (PAPS) is not attenuated, or a treated matter of the transformant (a), wherein the treated matter of transformant (a) is a matter in which the cell plasma membrane of the transformant (a) is substance permeable, and wherein the transformant (a) or the treated matter thereof is capable of producing PAPS;
• and
• (1-2) conducting a reaction for producing PAPS by using a reaction solution containing ATP or an ATP source, a sulfate ion source, and the transformant (a) or the treated matter of transformant (a), wherein the enzyme related to degradation of PAPS is not heat inactivated;
• and
• wherein the method further comprises conducting sulfation by a transformant comprising a gene encoding sulfotransferase, which is introduced thereinto in an expressible manner, or a treated matter or extract of the transformant comprising a gene encoding sulfotransferase.