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US 12,264,179 B2Granted
Epstein-Barr Virus antigen constructs
AssigneeGlaxosmithkline Biologicals SA
InventorsStefania Capone, Antonella Folgori, Armin Lahm, Benjamin Wizel
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Abstract
The invention provides Epstein-Barr Virus antigen polynucleotides, polypeptides and vectors; as well as immunogenic compositions comprising the same. It includes the use of Epstein-Barr Virus antigen constructs to produce vaccines for treating and preventing Epstein-Barr Virus infections and Epstein-Barr Virus-associated diseases, such as multiple sclerosis, rheumatoid arthritis and systemic lupus erythematosus.
Claims44
A nucleic acid comprising:
- (A) a polynucleotide encoding a polypeptide, the polypeptide comprising:
- (a) an Epstein-Barr Virus (EBV) latent membrane protein 1 (LMP1) fragment, the EBV LMP1 fragment comprising one or more of SEQ ID NOs:
- 2-5;
- (b) an EBV latent membrane protein 2 (LMP2) fragment, the EBV LMP2 fragment comprising one or more of SEQ ID NOs:
- 7-10;
- (c) an EBV nuclear antigen 1 (EBNA1) fragment, the EBNA1 fragment comprising at least 8 amino acids of SEQ ID NO:
- 11;
- (d) an EBV nuclear antigen 3A (EBNA3A) fragment, the EBNA3A fragment comprising one or more of SEQ ID NOs:
- 14-20;
- or
- (e) an EBV ZEBRA fragment, the EBV ZEBRA fragment comprising one or both of SEQ ID NO:
- 22 or SEQ ID NO:
- 23;
- wherein the polypeptide is at least 80% identical to SEQ ID NO:
- 24 or SEQ ID NO:
- 26;
- and
- (B) a sequence that is capable of directing expression of the polypeptide in a host cell;
- wherein the polynucleotide is operatively linked to the sequence that is capable of directing expression of said polypeptide in the host cell.
16 more elements
A nucleic acid comprising:
- (A) a polynucleotide encoding a polypeptide, the polypeptide comprising SEQ ID NO:
- 24 or SEQ ID NO:
- 26;
- and
- (B) a sequence that is capable of directing expression of the polypeptide in a host cell;
- wherein the polynucleotide is operatively linked to the sequence that is capable of directing expression of said polypeptide in the host cell.
3 more elements
Dependencies are inferred from the claim text.