Manufacturing method of the operation pipe

A manufacturing method of an operation pipe for operating target components, comprising:

• providing a hollow pipe having a closable open end for supplying a sample containing the target components on one side and a closed end on the other side, and having an operation pipe portion on the open end side and a recovery pipe portion on the closed end side;
• filling a recovery medium in the recovery pipe portion so that a first gel layer and a first aqueous liquid layer which is in contact with the closed end are multi-layered, wherein the first aqueous liquid layer in contact with the closed end has a predetermined volume, a layer length of the first aqueous liquid layer in contact with the closed end varies with variations in an inner diameter of the hollow pipe or a length of the hollow pipe, and a volume of the first aqueous liquid layer in contact with the closed end is 50 μL-300 μL, and a layer length of the first gel layer is determined by a length in the longitudinal direction of the hollow pipe;
• filling an operation medium in the operation pipe portion so that second gel layers and second aqueous liquid layers are alternately multi-layered in the longitudinal direction of the hollow pipe, wherein a layer length of each of the second gel layers and a layer length of each of the second aqueous liquid layers are determined by the length in the longitudinal direction of the hollow pipe.