Opto-fluidic array for radical protein foot-printing
AssigneeGennext Technologies, Inc.
InventorsScot Randy Weinberger, Robert Wallace Egan, Jeffrey Johnathan Persoff, Emily Elizabeth Chea, Tan Huu Bui, David Allan Holman
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Abstract
Systems and methods of in vivo and in vitro radical protein foot-printing using an opto-fluidic array are presented. These teachings may be used to, for example, study three-dimensional protein structure or bio-kinetics. Radical dosimetry including an optional intrinsic standard is used. Real-time feedback based on an internal standard provides comparability between different experiments and in vivo and in vitro analysis results in data that is representative of actual biological conditions.
Claims16
An opto-fluidic chip for radical protein footprinting comprising:
- a substrate;
- a first port, a second port, and a third port each defined into the substrate;
- a mixing zone comprising a first cavity defined within the substrate and in fluid communication with both the second and third ports, wherein mixing is performed within the first cavity;
- a photolysis zone comprising a second cavity defined within the substrate and in fluid communication with the mixing zone, the second cavity being bounded by first and second opposing transparent sides, the second cavity comprising a channel configured in a serpentine pattern, wherein photolysis is performed within the second cavity;
- and
- a dosimetry zone comprising a third cavity defined within the substrate and in fluid communication with the photolysis zone and in further fluid communication with the first port, the third cavity being bounded by first and second opposing sides wherein one of the first and second sides of the third cavity is optically transparent, and wherein contents of the third cavity are photometrically probed;
- wherein
- the photolysis zone is arranged between the mixing zone and the dosimetry zone.
An opto-fluidic system for radical protein footprinting comprising:
- a chip including a substrate and a first port, a second port, and a third port each defined into the substrate, the chip further including
- a mixing zone comprising a first cavity defined within the substrate and in fluid communication with both the second and third ports, wherein mixing is performed within the first cavity,
- a photolysis zone comprising a second cavity defined within the substrate and in fluid communication with the mixing zone, the second cavity being bounded by first and second opposing transparent sides, the second cavity comprising a channel configured in a serpentine pattern, wherein photolysis is performed within the second cavity;
- a dosimetry zone comprising a third cavity defined within the substrate and in fluid communication with the photolysis zone and in further fluid communication with the first port, the third cavity being bounded by first and second opposing sides wherein one of the first and second sides of the third cavity is optically transparent, wherein contents of the third cavity are photometrically probed and wherein the photolysis zone is arranged between the mixing zone and the dosimetry zone;
- and
- a microfluidics system in fluid communication with both the second and third ports.
Dependencies are inferred from the claim text.